Crosby initially led the club on an interim basis after former boss Nigel Adkins was dismissed in February 2025 before being appointed on a permanent deal in May.
He earned six wins and five draws from 13 games in charge of Tranmere at the tail end of last season as they avoided relegation by finishing 20th and nine points clear of the drop zone.
Their recent form has taken a drastic turn in comparison to their finish last term, however, with the club winning just twice since the turn of the year.
Following their 2-0 victory at Harrogate on New Year’s Day, Tranmere have picked up three points from a possible 33.
During that period, the gap between themselves and the bottom two has decreased from 15 to eight points, while they have conceded 10 goals in their past three games, including a 5-0 hammering by Notts County on 21 February.
Prior to his sacking, Tranmere Rovers’ Official Supporters’ Club had called, external for an “immediate change” to the managerial team, saying Crosby’s position was “untenable”.